2.2     Next, configure the lasers of a confocal microscope to 405 nanometers, 488 nanometers for unconverted Dendra2 excitation, and 561 nanometers [1]. Turn on the lasers and allow approximately 10 minutes for stabilization [2]. Authors: Please create screen capture videos of the shots labeled as SCREEN, create a screenshot summary, and upload the files to your project page as soon as possible: https://review.jove.com/account/file-uploader?src=21126788
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2.2.1.    SCREEN: Talent adjusting the laser configuration on the software. [0:00-0:09]

2.2.2.    SCREEN: Talent switching on the lasers. [0:10-0:24]

2.4.     Visualize the cells using higher magnification and resolution for mitochondrial imaging [1]. Open the drop-down menu in the top-left corner and select the FRAP (frapp) options [2].
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2.4.1.    SCOPE/SCREEN: Image of the mitochondria at high resolution is being seen.[0:10-0:27]
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2.4.2.    SCREEN: Software interface with drop-down menu open and FRAP option selected.[0:00-0:07]