Screen recordings in .mp4 format are labeled according to the script section number. For all recorded steps (except 5.2.1, 5.2.2 and 5.3.1) the example chosen for screen recording was HC brain slices with LFP detection in analysis. 

4.4.2:  Talent aligning the brain slices on the HD-MEA chip.

4.4.3a: Talent aspirating solutions from the chip
4.4.3b: Solution-free chip still image
NOTE: 4.4.3 a and b could be combined together into one video segment or just keep 4.4.3a. 4.4.3b is showing the slice without aspirator in frame. 

4.5.1:  Talent securing the slice with the anchor.

4.5.2:  Talent gently adding solution to the chip reservoir
NOTE: This screen recording could then cut to the actual video of us turning on the perfusion system live, but it is necessary to show us first adding the solution to the reservoir. The other option is us adding solution to the reservoir and turning the perfusion system on both in the live shot. 

4.6.1:  The Brainwave software is being started. File is being clicked followed by New Recording Session.

4.6.2:  Recording Frequency is being set to 1 Hz, Sampling Frequency is being set to 14 kHz per electrode. Record is being pressed.

4.7.1:  The light imaging of the acute brain slices is being captured.
NOTE: Both 4.7.1 a and b show the image acquisition, both images are used for alignment later. Either choose 4.7.1a alone or both. 

5.2.1:  (same as 4.6.1) The Brainwave software is being started. File is being clicked followed by New Recording Session. 

5.2.2:  The recording Frequency is being set to 50 Hz, sampling Frequency is being set to 18 kHz per electrode.

5.3.1:  The spontaneous firing activity or pharmacologically induced responses are being recorded. 

7.1.2a:  A recorded raw data file is being opened. 
7.1.2b:  Analysis is being selected, LFP Detection is being clicked
NOTE: Due to time needed to open a raw recorded data file, this video was broken into two, please use both for one step. 

7.2.1:  For hippocampus and olfactory bulb circuit recordings, the Advanced Workspace option for structural light image import from the stereomicroscope into the detected event file is being integrated.

7.2.2:  The structural layers for examining large-scale HC circuitry are being created.
NOTE: Due to time needed to create all clusters, this video was broken into two, please use both for one step. 

7.3.1:  A custom-written Python script to read the .bxr file is being executed.

7.3.2:  The spike trains from iPSC network recordings and LFP event trains from HC, and OB brain slice circuit recordings are being extracted. 
NOTE: This is just an example from HC brain slices

7.4.1:  The resultant event train data with spatiotemporal information is being saved in .npy file format.

7.5.1:  The cross-covariance between pairs of active electrodes in the 64 x 64 array is being calculated using time series data from the .brw file.

7.5.2:  A dynamic graph file is being created from connectivity matrices.

7.6.1a:  The data laboratory is being opened in the Gephi program. 
7.6.1b:  Geo Layout is being applied for spatial mapping. Parameter constraints are being set on Degree Range and Edge Weight
NOTE: Due to time needed to refine parameters, this video was broken into two, please use both for one step. 

7.6.2a and 7.6.2b:  Nodal Color, Edge Size, and Degree Size are being assigned.
NOTE: Due to time needed to refine parameters, this video was broken into two, please use both for one step.