Tilt angle specification
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Tilt to
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Always positive!
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End at
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Always negative!
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Run Series in two directions
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Always selected, set pre-tilt for lamellae.
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Delay time after tilting
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3 seconds for now, might change in th future.
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Use dose-symmetric scheme
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Always selected.
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Dose-symmetric Setup
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Scroll down this page.
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Size, Image Shift and recentering Specifications
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Limit Image shift to
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15 um at start, dont change!
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Nothing else selected.
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Beam Intensity or Exposure Control
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Keep beam intensity constant
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Always on.
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| Nothing else selected. |
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Camera and Acquisition Parameters
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EF-CCD
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Selected.
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| Nothing else selected. |
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Autofocus Control
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Defocus target
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Set for each position untill we have an automated solution from David.
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Beam Tilt
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4 mrad, 10 mrad for VPP Tomo.
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Iteration threshold
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0.1 um for high-resolution subtomo, something else for other Tomo: how much focus spread through a tiltseries is ok?
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Do autofocus at least every x degrees
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0.1
um for high-resolution subtomo (autofocus each tilt), something else
for other Tomo: how much focus spread through a tiltseries is ok? |
Stop if autofocus change exceeds
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10 um seems legit....
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Initial and Final Actions
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Nothing selected!
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Tracking Control Parameters
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Wait for drift to settle
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Not needed anymore on K3 and we use a 3 sec wait after tilt time allready.
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Get tracking image when error in X/Y prediction is > X % of image size
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0 % for high-resolution subtomo
(track each tilt), something else (the default 5%?) for other Tomo: how
much image shift between tilts through a tiltseries is ok? |
Track
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Before
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Align only with tracking images, not Record images
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Do never select this!
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Align with Preview befor getting new track reference
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??????? don't know.
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Get new track reference if record alignment differs by > X %
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??????? don't know. |
