Run batch tomograms
Goal:
- Acquire all selected and setup batch positions with the world famous Hagen-scheme.
Checks
- Autoloader turbo off?
- Enough disk space on Gatan PC X: drive?
- Objective aperture in and centered?
- Astigmatism ok?
- Dose rate ok?
- Frame saving on?
- Column valves open?
- Enough nitrogen in the tank?
The Krios uses 25 to 30 liters of liquid nitrogen per day, the big tank is about 200 liters
At the level shown here, just replace the tank.
Settings
SerialEM:
- Macro menu, Edit 10.
Run
SerialEM file menu, save the log file, then select
continuous saving for the log file. This is needed for trouble shooting.
SerialEM:
- Navigator menu, Acquire at points.
- Select Primary task Run macro 10.
- Select Close column valves at end.
- Select Send email at end.
- Nothing else selected!
GO!