Run SPA on holes

Goal:

Acquire frame stacks on all selected holes

Checks

Emai address set?
Autoloader turbo off?
Enough disk space on Gatan PC X: drive?
Objective aperture in and centered?
Astigmatism ok?
Dose rate and dose ok?
Frame saving on?
Column valves open?
Hole centering image in buffer P?
Enough nitrogen in the tank?

The Krios uses 25 to 30 liters of liquid nitrogen per day, the big tank is about 200 liters

At the level shown here, just replace the tank.

Settings

SerialEM:

Macro menu, Edit 5.

driftcrit                    Drift in A/sec, adjust this according to frame time, less than 1 pixel drift per frame.
driftinterval              3 seconds works nice
drifttimes                 After this amount of measurements the macro simply continues.

Run

SerialEM:

Navigator menu, Acquire at points.
Select Primary task Run script SPAK2aligntohole.
Select Close column valves at end.
Select Send email at end.
Nothing else selected!

GO!