Mapping Grids

Goal:

Setup microscope.
Start SerialEM macro to automatically:
- Wait some time for the loader to cool down again.
- Do AutoLoader Inventory.
- Map all grids found by the inventory at lowest magnification.

Titan UI:

Column valves must be closed!
Condenser 2 aperture 50 micrometer should be in.
Objective aperture must be out!

SerialEM:

Set your email address, menu Tilt Series/Set Email Address.
Navigator, Open imaging States.
Load imaging state, double click on "Grid map" (EFTEM LM 135, spot 6).

Check in Titan UI if the setting is ok, mag should me LM 135!!!

Open column valves in SerialEM:
Titan FluCam viewer, Insert Screen, check if the beam is centered around the GIF entrance aperture (small green circle).
Insert the beam stop halfway in with , things should now look like this (the right edge is not a problem):

Take a record image in SerialEM, image should look like this:
FluCam viewer, retract the beam stop with .

SerialEM Navigator menu, Open.
SerialEM Navigator menu, Montaging & Grids, Setup Full Montage .

Do not select "Skip this dialog in future (re-enable in file menu)"!

Macro menu, Edit 1, change the delay time if needed (depends on autoloader temperature, Krios 2 +/- 10 min).
Close column valves.

Set your email address in menu Tilt Series/Set email address.
Run Macro 1, takes a while.

Save Navigator file, Navigator menu, Save.
Choose the grid you want to image, select the grid in the Titan UI AutoLoader control panel, click load (any loaded grid will automatically be unloaded).