Krios 2 Quantum K3

Introduction

Manual

Microscope status
Liquid nitrogen
Unloading

Load & map grids

Loading
Map grids

K3 single particle

Low Dose single particle screening
Map grid squares
Setup grid squares
Tune GIF & gain reference
Microscope tuning
Low Dose single particle
Run

Warp

K3 Tomo

Map grid squares
Low Dose tomography
Tune GIF & gain reference
Microscope tuning
Virtual Tomo maps setup
Manually setting up positions
Run

Problems

Lens board errors
Restart software
Water failure
Large black vertical stripe in K3 images
Gatan K3 errors (Health Status not green)


Wim Hagen 2020