Head: Univ. Prof. Mag. Dr. Markus Hengstschläger
Head of Clinical Section: Assoc. Prof. PD Dr. Franco Laccone	

				
			Hannes Steinkellner, Mag. Dr.

Währinger Straße 10, 1090 Vienna, Austria
T: +43 (0)1 40160-56534
F: +43 (0)1 40160-956531
hannes.steinkellner@meduniwien.ac.at

Vienna, 20.01.2020	
Dear Dr. Xiaoyan Cao,
please find enclosed a revised version of the manuscript now entitled:

“Employing an electrochemiluminescence based assay for MeCP2 protein variants.”

In this manuscript we present the development and validation of an electrochemiluminescence-based immunoassay (ECLIA) to quantify both endogenous as well as recombinant MeCP2 protein levels in mice brain tissues, as well as in cell culture. This method is a very useful tool in developing novel strategies for the treatment of Rett Syndrome, a neurodevelopmental disease, brought about by mutations in the coding region of the MeCP2 protein.
We have addressed the reviewers’ comments to the best of our ability and incorporated the appropriate modifications into our manuscript. We have also obtained an explicit permission from Meso Scale Discovery to include the adapted figure 1 in our paper. The corresponding email has been attached.

We would very much appreciate your consideration for a publication in JoVE.

With kind regards,
Hannes Steinkellner