Robert W. O’Rourke, MD
  Professor of Surgery
  Division of Minimally Invasive Surgery
  Section of General Surgery

  Department of Surgery
  1500 East Medical Center Drive 
  2210 Taubman Center, SPC 5354
  Ann Arbor, MI 48109-5354
  (734) 936-7607
 
 rorourke@umich.edu

 
	
August 19, 2019

Phillip Steindel, Ph.D.
Review Editor, JoVE

Dear Dr. Steindel and Editors and Reviewers of J Vis Exp,

Please accept this submission of our revised manuscript “A human 3D extracellular matrix-adipocyte culture model for studying matrix-cell metabolic crosstalk”, by authors Flesher et al., for consideration for publication in J Vis Exp.

We appreciate the reviewers’ thoughtful, thorough, and professional critiques. We have significantly revised the manuscript, added substantial additional data, and addressed each concern to the best of our ability in the revised manuscript and the point-by-point response below. We feel that these changes greatly strengthen the manuscript and we thank the reviewers for their suggestions.

We have included an email from Oxford University Press permitting republication of figure 4 of this revised manuscript, which was originally published in Baker et al., J Clin Exp Metab 2017.

The authors have no conflicts of interest. This work has not been previously published. 
We appreciate your attention to this manuscript and we look forward to your decision.

Sincerely,

Robert W. O’Rourke, MD