Response to editors
Manuscript #JoVE59452
“A Method to Enhance Engraftment of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes via Transient Inhibition of Rho Kinase Activity”
By Meng Zhao, et al
We appreciate the careful and constructive review given to our manuscript. We have revised the manuscript to address the comments from editor and reviewers. All changes were marked in red. A point by point response is as follows.
Editorial comments:

The manuscript has been modified and the updated manuscript, 59452_R1.docx, is attached and located in your Editorial Manager account. Please use the updated version to make your revisions.

1. Please take this opportunity to thoroughly proofread the manuscript to ensure that there are no spelling or grammar issues.
Response: We have thoroughly proofread the manuscript to check for spelling or grammar issues.

2. The manuscript has been rearranged and the Figure Legend has been moved before Discussion. Please adjust the numbering of all references and ensure that all references are numbered in the order of the appearance in the manuscript.
Response: Thank you! We have adjusted the numbering of all references and ensured that all references are numbered in the order of the appearance in the manuscript.

3. JoVE cannot publish manuscripts containing commercial language. This includes company names before an instrument or reagent. Please remove all commercial language from your manuscript and use generic terms instead. All commercial products should be sufficiently referenced in the Table of Materials and Reagents. Examples of commercial language in your manuscript include mTeSR, Matrigel, Pluronic, Olympus, Fluoview, etc.
Response: We have removed all commercial language from our manuscript and used generic terms instead. All commercial products can be sufficiently referenced in the Table of supplement.
4. Please remove trademark (™) and registered (®) symbols from the Table of Equipment and Materials.
Response: We have removed all the trademark (™) and registered (®) symbols.

5. Please use standard SI unit symbols and prefixes such as µm, µL, mL, L, g, m, etc.
Response: We checked the entire manuscript to ensure that we used standard SI unit symbols and prefixes.

6. Please use h, min, s for time units.
Response: We checked the entire manuscript to ensure that we used h, min, s for time units.

7. Please use a single space between numerical values and their units.
Response: We have correct this in the revised manuscript.

8. Step 2.1: Please specify the culture condition.                                                                                                                   
Response: The culture condition has been specified in Step 2.1.

9. 3.2: Please convert centrifuge speeds to centrifugal force (x g) instead of revolutions per minute (rpm).
Response: All centrifuge speeds in the entire manuscript have been converted to centrifugal force (x g).

10. Discuss maintenance of sterile conditions during survival surgery.
Response: The maintenance of sterile conditions during survival surgery has been discussed in surgery section (section 4, line 188-189).

11. Please specify that the animal is not left unattended until it has regained sufficient consciousness to maintain sternal recumbency.
Response: We added this information to Section 4.7 (line 226-228).
12. Please specify that the animal that has undergone surgery is not returned to the company of other animals until fully recovered.
Response: We added this information to Section 4.7 (line 226-228).

13. Please remove all headers from Representative Results.
Response: All headers have been removed from Representative Results.

14. Please do not abbreviate journal titles for all references.
Response: The abbreviations were generated by Endnote automatically. We will manually correct this before production of this manuscript.  

15. Figure 3C: Please define the scale bar.
Response: We defined the scar bar in figure legend.