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<article dtd-version="3.0" article-type="Invited Methods Article - JoVE Produced Video">
  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher">jove</journal-id>
      <journal-id journal-id-type="email">editorial-office@jove.com</journal-id>
      <journal-title-group>
        <journal-title>Journal of Visualized Experiments</journal-title>
      </journal-title-group>
      <issn pub-type="ppub">1940-087X</issn>
      <issn pub-type="epub"></issn>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="publisher-id">JoVE58669</article-id>
      <article-id pub-id-type="manuscript">JoVE58669</article-id>
      <article-categories>
        <subj-group subj-group-type="Article Type">
          <subject>Invited Methods Article - JoVE Produced Video</subject>
        </subj-group>
        <subj-group subj-group-type="Category">
          <subject>JoVE Biochemistry</subject>
        </subj-group>
        <subj-group subj-group-type="Classification">
          <subject>Chemistry Techniques, Analytical</subject>
          <subject>Electrophoresis</subject>
          <subject>Electrophoresis, Polyacrylamide Gel</subject>
          <subject>Analytical, Diagnostic and Therapeutic Techniques and Equipment</subject>
          <subject>Investigative Techniques</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title>Fluorescent Silver Staining of Proteins in Polyacrylamide Gels</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <role content-type="1" />
          <name>
            <surname>Wong</surname>
            <given-names>Alex.  Y.H.</given-names>
          </name>
          <email>alex.wong@ki.se</email>
          <xref ref-type="aff" rid="aff1" />
        </contrib>
        <contrib contrib-type="author">
          <role content-type="2" />
          <name>
            <surname>Xie</surname>
            <given-names>Sheng</given-names>
          </name>
          <email>Sheng.xie@ki.se</email>
          <xref ref-type="aff" rid="aff2" />
        </contrib>
        <contrib contrib-type="author">
          <role content-type="3" />
          <name>
            <surname>Tang</surname>
            <given-names>Ben  Zhong</given-names>
          </name>
          <email>tangbenz@ust.hk</email>
          <xref ref-type="aff" rid="aff3" />
        </contrib>
        <contrib contrib-type="author" corresp="yes">
          <role content-type="4" />
          <name>
            <surname>Chen</surname>
            <given-names>Sijie</given-names>
            <prefix>Dr.</prefix>
          </name>
          <email>sijie.chen@ki.se</email>
          <xref ref-type="aff" rid="aff4" />
        </contrib>
        <contrib contrib-type="editor">
          <name>
            <surname>Navani</surname>
            <given-names>Dipesh</given-names>
          </name>
          <degrees>Ph.D.</degrees>
          <email>dipesh.n@jove.com</email>
          <xref ref-type="aff" rid="aff5" />
        </contrib>
        <aff id="aff1">
          <institution>Karolinska Institutet</institution>
          <addr-line content-type="addrline1">Units 608-613 Building 15 Science Park, 15W Science Park W Ave</addr-line>
          <country>HONG KONG</country>
          <addr-line content-type="city">Hong Kong</addr-line>
          <addr-line content-type="zipcode">0000</addr-line>
          <institution>Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong</institution>
        </aff>
        <aff id="aff2">
          <institution>Karolinska Institutet</institution>
          <addr-line content-type="addrline1">Units 608-613 Building 15 Science Park, 15W Science Park W Ave</addr-line>
          <country>HONG KONG</country>
          <addr-line content-type="city">Hong Kong</addr-line>
          <addr-line content-type="zipcode">0000</addr-line>
          <institution>Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong</institution>
        </aff>
        <aff id="aff3">
          <institution>Hong Kong University of Science and Technology</institution>
          <addr-line content-type="addrline1">The Hong Kong University of Science and Technology, Kowloon, Hong Kong.</addr-line>
          <country>HONG KONG</country>
          <addr-line content-type="city">Hong Kong</addr-line>
          <addr-line content-type="zipcode">0000</addr-line>
          <institution>Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and</institution>
        </aff>
        <aff id="aff4">
          <country>SWEDEN</country>
        </aff>
        <aff id="aff5">
          <institution>JoVE</institution>
          <addr-line>1 Alewife Center</addr-line>
          <addr-line>Suite 200</addr-line>
          <addr-line>Cambridge, MA  02140</addr-line>
          <country>UNITED STATES</country>
        </aff>
      </contrib-group>
      <history>
        <date date-type="received">
          <day>25</day>
          <month>06</month>
          <year>2018</year>
        </date>
        <date date-type="rev-recd">
          <day>03</day>
          <month>08</month>
          <year>2018</year>
        </date>
        <date date-type="accepted">
          <day>10</day>
          <month>08</month>
          <year>2018</year>
        </date>
      </history>
      <abstract>
        <p>Silver staining is a colorimetric technique widely used to visualize protein bands in polyacrylamide gels following SDS-PAGE. The classic silver stains have certain drawbacks, such as high background staining, poor protein recovery, low reproducibility, narrow linear dynamic range for quantification and limited compatibility with mass spectrometry (MS). Now, with use of a fluorogenic Ag+ probe, TPE-4TA, we developed a fluorescent silver staining method for total protein visualization in polyacrylamide gels. This new stain avoids the troublesome silver reduction step in traditional silver stains. Moreover, the fluorescent silver stain demonstrates good reproducibility, sensitivity and linear quantification in protein detection, making it a useful and practical protein gel stain.</p>
      </abstract>
      <kwd-group>
        <kwd>Silver staining</kwd>
        <kwd>Fluorescent gel staining</kwd>
        <kwd>Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)</kwd>
        <kwd>Aggregation induced emission (AIE)</kwd>
        <kwd>Silver ion sensor</kwd>
        <kwd>Fluorogenic detection</kwd>
        <kwd>Proteomics</kwd>
      </kwd-group>
      <funding-group />
      <counts>
        <fig-count count="0" />
      </counts>
      <custom-meta-group>
        <custom-meta>
          <meta-name>Please indicate whether this article will be Standard Access or Open Access. </meta-name>
          <meta-value>Standard Access (US$2,400);</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>Please indicate the &lt;b&gt; city, state/province, and country&lt;/b&gt; where this article will be &lt;b&gt;filmed&lt;/b&gt;. Please do not use abbreviations.</meta-name>
          <meta-value>Hong Kong S.A.R</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>Black and White Image Count</meta-name>
          <meta-value>0</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>Color Image Count</meta-name>
          <meta-value>0</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>Acquisitions Editor</meta-name>
          <meta-value>Benjamin Werth</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>Assistant who entered lead</meta-name>
          <meta-value>Meaghan McCarthy</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>Additional Video Charge</meta-name>
          <meta-value>Author wants to downgrade to standard access</meta-value>
        </custom-meta>
        <custom-meta>
          <meta-name>ALA Access Type</meta-name>
          <meta-value>JPV Standard Access ($2400)</meta-value>
        </custom-meta>
      </custom-meta-group>
    </article-meta>
  </front>
  <body>
    <table-wrap>
      <graphic xlink:href="JoVE_Materials.xls" xmlns:xlink="http://www.w3.org/1999/xlink" />
    </table-wrap>
    <fig>
      <label>Figure 1</label>
      <graphic xlink:href="Figure 1.psd" xmlns:xlink="http://www.w3.org/1999/xlink" />
    </fig>
    <fig>
      <label>Figure 2</label>
      <graphic xlink:href="figure 2.psd" xmlns:xlink="http://www.w3.org/1999/xlink" />
    </fig>
    <fig>
      <label>Figure 3</label>
      <graphic xlink:href="Figure 3.psd" xmlns:xlink="http://www.w3.org/1999/xlink" />
    </fig>
    <fig>
      <label>Figure 4</label>
      <graphic xlink:href="figure 4.psd" xmlns:xlink="http://www.w3.org/1999/xlink" />
    </fig>
    <table-wrap>
      <label>Table of Materials</label>
      <graphic xlink:href="JoVE_Materials revised.xls" xmlns:xlink="http://www.w3.org/1999/xlink" />
    </table-wrap>
  </body>
</article>