LOCUS       Exported                5620 bp ds-DNA     circular SYN 25-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
COMMENT     LOCUS pET-28 a (+) 5369 bp DNA SYN 17-Apr-2014 DEFINITION pET-28 a 
            (+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial 
            sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="synthetic DNA construct"
                     /label=pET-28
     gene            complement(179..442)
                     /label=MinE
                     /note="MinE"
     misc_feature    complement(443..520)
                     /label=LINKER
                     /note="LINKER"
     misc_feature    521..538
                     /label=6xHis
                     /note="6xHis"
     misc_feature    complement(558..574)
                     /label=T7_transl_en_RBS
                     /note="T7_transl_en_RBS"
     misc_feature    complement(592..619)
                     /label=lacO
                     /note="lacO"
     promoter        complement(619..637)
                     /label=T7_promoter
                     /note="T7_promoter"
     gene            complement(669..932)
                     /label=tet
                     /note="tet(564-300)"
     misc_feature    1015..2106
                     /label=lacI
                     /note="lacI"
     gene            complement(2165..2440)
                     /label=tet
                     /note="tet(852-576)"
     misc_feature    2915..3106
                     /label=ROP
                     /note="ROP"
     rep_origin      complement(3521..4140)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="pBR322_origin"
     gene            4246..5061
                     /label=KanR2
                     /note="KanR2"
     rep_origin      complement(5286..5592)
                     /direction=LEFT
                     /label=f1_origin
                     /note="f1_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt
      181 atttcagctc ttctgcttcc ggtaaggtca cgttcagctc aagaatagaa atatcgccat
      241 ctttttgctc aagctgtacg gttaccatct caggatcaat ttgtacgtat ttacaaatga
      301 cctcaagaat atctttacgc aactgcggca gataatgcgg ttctgcatcg ctgcgacggc
      361 gttcagcaac aataatctgc agccgttctt ttgcaatgtt ggctgtgttt ttcttccgcg
      421 agagaaagaa atcgagtaat gcgaattcgg atccgcgacc catttgctgt ccaccagtca
      481 tgctagccat atggctgccg cgcggcacca ggccgctgct gtgatgatga tgatgatggc
      541 tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg
      601 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga
      661 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      721 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
      781 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
      841 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
      901 gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg
      961 cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
     1021 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     1081 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     1141 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     1201 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
     1261 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
     1321 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
     1381 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
     1441 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
     1501 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
     1561 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
     1621 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
     1681 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
     1741 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
     1801 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
     1861 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     1921 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     1981 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     2041 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     2101 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc
     2161 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     2221 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     2281 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     2341 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     2401 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat
     2461 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca
     2521 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac
     2581 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa
     2641 gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg
     2701 tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg
     2761 gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg
     2821 ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc
     2881 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct
     2941 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
     3001 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
     3061 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     3121 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     3181 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     3241 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata
     3301 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     3361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3961 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4201 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     4261 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg
     4321 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     4381 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     4441 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     4501 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
     4561 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     4621 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     4681 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     4741 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     4801 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     4861 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     4921 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     4981 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     5041 atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta
     5101 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt
     5161 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     5221 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     5281 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     5341 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     5401 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     5461 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     5521 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     5581 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//
