LOCUS       Exported                6877 bp ds-DNA     circular SYN 25-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
COMMENT     Vector pET 28a (+)
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(179..988)
                     /label=MinD
                     /note="MinD"
     misc_feature    complement(179..226)
                     /label=MTS
                     /note="MTS"
     misc_feature    989..994
                     /label=Linker
                     /note="Linker"
     CDS             complement(995..1705)
                     /codon_start=1
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_structure  1706..1777
                     /label=Linker
                     /note="Linker"
     misc_structure  1778..1795
                     /label=6xHis Tag
                     /note="6xHis Tag"
     misc_feature    1796..178
                     /label=pET-28
     gene            complement(1926..2189)
                     /label=tet(564-300)
                     /note="tet(564-300)"
     misc_feature    2272..3363
                     /label=lacI
                     /note="lacI"
     gene            complement(3422..3697)
                     /label=tet(852-576)
                     /note="tet(852-576)"
     misc_feature    4172..4363
                     /label=ROP
                     /note="ROP"
     rep_origin      complement(4778..5397)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="pBR322_origin"
     gene            5503..6318
                     /label=KanR2
                     /note="KanR2"
     rep_origin      complement(6543..6849)
                     /direction=LEFT
                     /label=f1_origin
                     /note="f1_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt
      181 atcctccgaa caagcgtttg aggaagcctt tcttctcttc ttcaatgaag cggaaaggac
      241 gttcttctcc caacagacgt tctacggtat ctgcgtaggc tttacccgca tcggcgttaa
      301 tgtcgagaat gaccggttca ccctggttag aggcgcgcaa tactgattga tcctctggga
      361 tcacgccgac gagtttgatg cgcaggatct ccagcacatc ttccatgctc agcatgtcac
      421 ctctgcttac gcggcctggg ttatagcgcg ttaacagcag gtgctcttta ataggctctt
      481 cgccattttc tgcgcggcgt gatttcgacg ccagaatgcc taaaatacgg tcagagtcgc
      541 gtactgagga gacttccggg ttggtggtaa taatggcttc gtctgcaaaa tagagtgcca
      601 ttaacgcacc ggtttcaatc cctgccgggg agtcacaaac gataaattca aaatccatcg
      661 ctttcagatc atcaagaact ttggcgaccc cttcacgggt gagggcatct ttatcgcgtg
      721 tttgcgatgc cggcagaata tagagatttt cagtacgctt atctttaatt aacgcctgat
      781 ttagcgttgc atcgccctga atgacgttga cgaaatcgta aacgacccgg cgttcacaac
      841 ccataatcag gtcgagatta cgcaggccga tatcaaaatc tatcacgaca gttttctttc
      901 ccttctgggc caaaccagtg gcgatggccg cgctggaggt tgtcttacca acaccccctt
      961 tgcccgaagt aacaacaata atgcgtgcga attccttgta cagctcgtcc atgccaccac
     1021 ccaggttaga gtatttagca acagcaactt cacgctgaac aacgtaggtt tcgttgtcag
     1081 attcttcgat acgttccaga cggtggtcaa cagcgtgaac acccggcatt ttgatgttac
     1141 caacggtttt tttagaacgg taggtggtaa cgaagttgca gtgcaggtga ccaccaccgt
     1201 caactttcag agcgatgtcg gtgtaaccac gcagaccacc gtcagccggg tacatcattt
     1261 cggtgttcgg ttcccaacct ttggtttttt tctgcataac cggaccgtta gacgggaagt
     1321 taacaccacg aactttcacg ttgtacacca gctcgccgtc ttccagagag gtgtcctggg
     1381 taacggtaac aacaccaccg tcttcgtaac gggtaacacg ttcccaggtg aagccctctg
     1441 ggaaagactg tttgaagaag tccgggatgt cagccgggta tttgatgaag gtacgcgaac
     1501 cgtacatgaa gctggtcgcg agtatgtcga acgcgaatgg gagcggacca ccttcgataa
     1561 ctttgatacg catggtctga acaccttcgt acggacgacc ttcaccttca ccggtgcatt
     1621 tgaactggtg accgttaaca gaaccttcca taacaacttt catacgcatg ttttctttga
     1681 tcagttcctc gcccttgctc accatggatc cgcgacccat ttgctgtcca ccagtcatgc
     1741 tagccatatg gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc
     1801 tgcccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
     1861 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc
     1921 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     1981 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     2041 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     2101 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     2161 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
     2221 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
     2281 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     2341 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     2401 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     2461 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     2521 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     2581 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     2641 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     2701 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     2761 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     2821 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     2881 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
     2941 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
     3001 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     3061 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     3121 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     3181 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     3241 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     3301 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3361 tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat
     3421 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     3481 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     3541 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     3601 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     3661 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat
     3721 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
     3781 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
     3841 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
     3901 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
     3961 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
     4021 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
     4081 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
     4141 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
     4201 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     4261 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     4321 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4441 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     4501 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
     4561 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     4621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5221 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5341 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5401 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca
     5461 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     5521 gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     5581 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
     5641 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     5701 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     5761 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
     5821 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     5881 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     5941 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     6001 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     6061 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     6121 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     6181 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     6241 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     6301 ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa
     6361 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa
     6421 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     6481 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     6541 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     6601 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     6661 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     6721 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     6781 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     6841 gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//
