// FiberQuant macro developed in the Bannister lab by Sidharth Tyagi
 macro "Batch Find Area" { 
     dir = getDirectory("C:\Users\Microscope\Desktop\Sid"); 
     //sets directory for image acquisition 
     list = getFileList(dir); 
     setBatchMode(true); 
     //enables batchmode
     for (i=0; i<list.length; i++) { 
        path = dir+list[i]; 
        open(path); 
        setAutoThreshold("Default");
        //run("Threshold...");
		setThreshold(0, 40);
        setOption("BlackBackground", false);
		run("Convert to Mask"); 
		//converts to binary, thresholded image
        run("Analyze Particles...", 
           "size=200-10000 circularity=0.10-1.00 show=Outlines display clear"); 
        path2 = dir+File.nameWithoutExtension; 
        saveAs("tiff", path2+"-bin.tif"); 
        close(); 
        saveAs("results", path2+"-results.xls"); 
     } 
  }